fc control  (R&D Systems)

Journal: bioRxiv

Article Title: Single injection of modified self-amplifying RNA encoding a CD19 bispecific T cell engager mediates long-term malignant B cell clearance

doi: 10.64898/2026.04.18.719371

Figure Lengend Snippet: (A) Design and timeline of the Nalm6 rechallenge study in NSG mice. (B) Progression-free survival of mice treated with blinatumomab protein, mRNA-BiTE, or saRNA-BiTE. Disease recurrence is defined as maintained flux > 1.5×10 6 on IVIS. Statistical significance was determined by the Logrank test. (C) Longitudinal tumor signal as measured on IVIS for individual mice in each treatment group in comparison to no tumor control mice. (D) Body weight change of mice within the first week after treatment. Data are presented as mean ± s.d. (n = 5 to 7 biological replicates). (E) Ex vivo killing efficiency of serum collected from treated mice over time in Nalm6 cell and primary human T cell co-cultures. Data are presented as geometric mean with the 95% confidence interval (left) or as curves for individual mice (right). (F) Serum human IFNγ levels on day 1 post treatment from animals in the different treatment groups. Data are presented as mean ± s.d. (n = 5 to 7 biological replicates). Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison.

Article Snippet: The media samples as well as blinatumomab protein control (MedChem Express) were loaded on a 10% Bis-Tris gel, separated by gel electrophoresis in MES buffer, and transferred onto nitrocellulose membrane.

Techniques: Comparison, Control, Ex Vivo

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus

doi: 10.1128/aac.01605-25

Figure Lengend Snippet: MAB_2736c protects purified ribosomes against linezolid. ( A ) Schematic of cell-free transcription/translation assay derived from E. coli . DHFR = Dihydrofolate reductase. ( B ) mNeonGreen fluorescence when co-expressed with DHFR in the presence of 25 μM, 50 μM, and 100 µM linezolid or DMSO. n = 5 independent samples. ( C ) mNeonGreen fluorescence when co-expressed with MAB_2736c in the presence of 25 μM, 50 μM, and 100 µM linezolid or DMSO. n = 5 independent samples. ( D ) Relative protein synthesis rate for mNeonGreen co-expressed with MAB_2736c or DHFR in the presence of 25 μM, 50 μM, or 100 μM linezolid, or DMSO. Rates were calculated from post-linezolid addition until 105 min elapsed. Data are presented as individual values along with mean +/− SD. P -values derived from Šídák’s multiple comparisons test after two-way ANOVA. n = 5 biological replicates.

Article Snippet: Plasmids carrying MAB_2736c or mNeonGreen compatible with the PURExpress in vitro protein synthesis kit were cloned into the manufacturer’s PURExpress DHFR Control Plasmid (E6800L, NEB) by swapping out DHFR with MAB_2736c (PCR amplified from ATC19977 gDNA) or mNeonGreen using standard restriction cloning with NdeI (R0111S, NEB) and PacI (R0527S, NEB).

Techniques: Purification, Derivative Assay, Fluorescence